Mechanism of o -aminophenol metabolism in human erythrocytes

Adenosine Metabolism in Human Erythrocytes

Magnesium metabolism of human and rabbit erythrocytes.

By STANLEY GINSBURG, JAMES G. Sxirni, FREEMAN M. GINSBURG, JACQUELINE Z. REARDON AND JERIIY K. AIKAWA T HE CURRENT PAUCITY of information concerning magnesium metabolism in erythrocytes is due in part to the lack of a reliable method for determining magnesium in red cells, and in part to the fact that a radioactive isotope of magnesium suitable for tracer studies has only recently become availa...

Metabolism of para-aminophenol by rat hepatocytes.

Autoxidation of para-aminophenol (PAP) has been proposed to account for the selective nephrotoxicity of this compound. However, other studies suggest that hepatic metabolites of PAP rather than the parent compound may be responsible for renal damage. These studies were designed to investigate PAP metabolism in isolated hepatocytes. We synthesized several proposed metabolites for analysis by HPL...

Mechanism of methaemoglobin reduction by human erythrocytes.

The time course of methaemoglobin reduction in human erythrocytes treated with nitrite was studied at pH 7.4, 37 degrees C, in the presence or absence of Methylene Blue, and the changes in methaemoglobin, intermediate haemoglobins and oxyhaemoglobin during the reaction were analysed by isoelectric-focusing on Ampholine/polyacrylamide-gel plates. In both cases, with or without the dye, the inter...

Role of aminotransferases in glutamate metabolism of human erythrocytes

Human erythrocytes require a continual supply of glutamate to support glutathione synthesis, but are unable to transport this amino acid across their cell membrane. Consequently, erythrocytes rely on de novo glutamate biosynthesis from α-ketoglutarate and glutamine to maintain intracellular levels of glutamate. Erythrocytic glutamate biosynthesis is catalyzed by three enzymes, alanine aminotran...